diff --git a/services/auth/basic.go b/services/auth/basic.go
index c8cb1735ee..eebdff2bad 100644
--- a/services/auth/basic.go
+++ b/services/auth/basic.go
@@ -5,6 +5,7 @@
 package auth
 
 import (
+	"errors"
 	"net/http"
 	"strings"
 
@@ -132,6 +133,16 @@ func (b *Basic) Verify(req *http.Request, w http.ResponseWriter, store DataStore
 		return nil, err
 	}
 
+	hashWebAuthn, err := auth_model.HasWebAuthnRegistrationsByUID(req.Context(), u.ID)
+	if err != nil {
+		log.Error("HasWebAuthnRegistrationsByUID: %v", err)
+		return nil, err
+	}
+
+	if hashWebAuthn {
+		return nil, errors.New("Basic authorization is not allowed while having security keys enrolled")
+	}
+
 	if skipper, ok := source.Cfg.(LocalTwoFASkipper); !ok || !skipper.IsSkipLocalTwoFA() {
 		if err := validateTOTP(req, u); err != nil {
 			return nil, err
diff --git a/tests/integration/api_twofa_test.go b/tests/integration/api_twofa_test.go
index 0bb20255a2..fb1d2badfc 100644
--- a/tests/integration/api_twofa_test.go
+++ b/tests/integration/api_twofa_test.go
@@ -15,6 +15,7 @@ import (
 	"code.gitea.io/gitea/tests"
 
 	"github.com/pquerna/otp/totp"
+	"github.com/stretchr/testify/assert"
 	"github.com/stretchr/testify/require"
 )
 
@@ -58,3 +59,24 @@ func TestAPITwoFactor(t *testing.T) {
 	req.Header.Set("X-Forgejo-OTP", passcode)
 	MakeRequest(t, req, http.StatusOK)
 }
+
+func TestAPIWebAuthn(t *testing.T) {
+	defer tests.PrepareTestEnv(t)()
+
+	user := unittest.AssertExistsAndLoadBean(t, &user_model.User{ID: 32})
+	unittest.AssertExistsAndLoadBean(t, &auth_model.WebAuthnCredential{UserID: user.ID})
+
+	req := NewRequest(t, "GET", "/api/v1/user")
+	req.SetBasicAuth(user.Name, "notpassword")
+
+	resp := MakeRequest(t, req, http.StatusUnauthorized)
+
+	type userResponse struct {
+		Message string `json:"message"`
+	}
+	var userParsed userResponse
+
+	DecodeJSON(t, resp, &userParsed)
+
+	assert.EqualValues(t, "Basic authorization is not allowed while having security keys enrolled", userParsed.Message)
+}